Antibacterial Activity of Citrus hystrix Extracts and Their Application in Foot Deodorant Spray Development
Keywords:
Citrus hystrix, Staphylococcus aureus, Herbal extractsAbstract
This research aimed to (1) investigate the antibacterial activity of kaffir lime (Citrus hystrix) leaf and peel extracts against Staphylococcus aureus, FT Isolate 1, and FT Isolate 2—bacteria isolated from individuals with foot odor—and (2) develop a herbal foot deodorant spray using the most effective extract. The antibacterial activity was assessed using the agar well diffusion method. The results showed that the peel extract exhibited greater inhibition against all three bacterial strains compared to the leaf extract. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the peel extract were both 125 µg/ml for all tested strains. The peel extract at the MIC level was then used to formulate a herbal foot spray. The final product was re-evaluated for antibacterial activity using the same method, and the results confirmed that the spray effectively inhibited the growth of all three bacterial strains. In conclusion, Citrus hystrix peel extract and its derived herbal spray demonstrated significant potential for controlling odor-causing bacteria on the feet.
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